Turn on mod_alias and mod_cgi

To setup cgi-bin access, you have to enable both the mod_alias and mod_cgi in /etc/lighttpd/lighttpd.conf:

server.modules += ( "mod_alias" )

include "mod_cgi.conf"

Setup cgi-bin access in localhost

Lighttpd installation came with a mod_cgi.conf in /etc/lighttpd. Check that file to make sure that it has:

alias.url = ( "/cgi-bin/" => "/var/www/localhost/cgi-bin/" )
$HTTP["url"] =~ "^/cgi-bin/" {
    # disable directory listings
    dir-listing.activate = "disable"
    # only allow cgi's in this directory
    cgi.assign = (
              ".pl"  => "/usr/bin/perl",
              ".cgi"  => "/usr/bin/perl"
    )
}

Check /etc/lighttpd/lighttpd.conf has the following line:

static-file.exclude-extensions = (".php", ".pl", ".cgi", ".fcgi")

Setup cgi-bin access in a virtual host:

Find out your virtual server configuration and append following text:

$HTTP["url"] =~ "/cgi-bin/" {
      cgi.assign = ( ".pl" => "/usr/bin/perl" )
  }

Here is complete my config code:

$HTTP["host"]  =~ "yourhost.com" {
  server.document-root = "/home/lighttpd/yourhost.com/http"
  server.errorlog            = "/var/log/lighttpd/yourhost.com/error.log"
  accesslog.filename         = "/var/log/lighttpd/yourhost.com/access.log"
  $HTTP["url"] =~ "/cgi-bin/" {
      cgi.assign = ( ".pl" => "/usr/bin/perl",
                            ".cgi" => "/usr/bin/perl")
  }
}